iScreen measures the following drugs and/or their metabolites at or below cut-off levels defined by the Australian standard AS 4760:2006, which describes procedures for the collection, detection and quantitation of drugs in oral fluid.
If you would like further information about these narcotics please view our drugs of abuse information page.
iScreen is a combination of individual tests, which undertake analysis for a specific component based on the principle of solid-phase immunoassay technology. Using this method a specific immunochemical reaction between antibodies and antigen is employed to analyse a corresponding compound within a biological matix.
The assay is based on competition between the drug conjugate and any free drug to bind with an antibody. If a drug is present in the specimen undergoing analysis, it competes with drug conjugate for antibody-dye conjugate, which is in limited supply. If the quantity of drug is equal to, or greater than, the cut-off levels of any of the drugs, it inhibits binding of the drug conjugate to the antibody.
This is why, as illustrated below, no test line is visible when a result is positive whereas the presence of a coloured band indicates a negative result.
Each iScreen drug test kit contains of the following inclusions:
The following instructions provide details on how to use iScreen to accurately analyse a saliva sample for drugs of abuse.
Allow the test device to come to room temperature (15 – 30 °C) prior to testing. Instruct the donor not to place anything in the mouth including food, drink, gum, or tobacco products for at least 10 minutes prior to collection.
Bring the pouch to room temperature before opening it. Remove the test and Cap from the sealed pouch
and use the test as soon as possible.
Remove the Sample Collector Protector from the collection Sponge. Instruct the donor to insert the
Sponge end of the collector into the mouth and actively swab the inside of the mouth and the top of the
tongue. As soon as the Sponge softens slightly, the donor should gently press the Sponge between the
tongue and teeth to ensure complete saturation.
The Sponge is saturated when no hard spots can be felt. Collect for a total of at least three (3) minutes before removing the Sponge. Remove the collector from the mouth.
Align the Red Arrow on the device with one of the White Marks on the Cap. Insert the collector vertically
into the Cap and press down firmly. Twist the Cap clockwise 180° until the Red Arrow lines up with the
other White Mark.
Place the test device horizontally on a clean and level surface with facing up.
Read results at 10 minutes. Do not read results after 1 hour.
If positive results are observed, secure Cap with security seal and send the device to a laboratory for
confirmation. The laboratory can access the reservoir through the Sample Port.
For detailed operating instructions, please refer to the Procedure Card.
All test lines appear. One colored line should be in the control region (C), and other apparent
colored line should be adjacent in the test region (Drug/T). This negative result indicates that the drug
concentration is below the detectable level or drug free.
One colored line appears in the control region (C). Any test line not appears in the test
region (Drug/T). This positive result indicates that the drug concentration is above the detectable level.
Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are
the most likely reasons for control line failure. Review the procedure and repeat the test using a new test
panel. If the problem persists, discontinue using the lot immediately and contact the manufacturer.
*Note: The shade of color in the test region (Drug/T) will vary, but it should be considered negative whenever there is even a faint colored line.
The following documents related to iScreen are available for viewing and download: